Bench-side reference

Cellenix

Six calculators for the math you redo every week: stock solutions, dilutions, antibody prep, serial series, seeding density, and media supplements. Everything runs in your browser as you type.

Stock solution prep

Enter the molarity you want, the molecular weight of your compound, and the volume you're making. Get the mass to weigh out.

Target concentration

Molecular weight

Final volume

Mass to weigh

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Molar concentration

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Dilution (C₁V₁ = C₂V₂)

Diluting a stock down to a working concentration. Enter your stock concentration, the concentration you want, and the final volume you need.

Stock concentration (C₁)

Desired concentration (C₂)

Final volume (V₂)

C₂ can't be higher than C₁ — you can't dilute up to a stronger concentration.

Volume of stock

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Volume of diluent

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Antibody dilution

Enter the dilution ratio off the datasheet (e.g. 1:500) and the final volume you need, and get exact pipetting volumes.

Dilution ratio — 1 : ___

Final volume needed

Antibody volume

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Diluent volume

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Serial dilution series

Set a starting concentration, a fold-dilution per step, how many tubes, and the volume in each tube. Get a full transfer table.

Starting concentration

Fold dilution per step

Number of tubes

Volume per tube (µL)

Tube	Concentration	Transfer in	Diluent

Cell seeding density

From your counted stock concentration, work out how much cell suspension and how much media go into each well — for one well or a whole plate.

Cells wanted per well

Counted stock concentration (cells/mL)

Final volume per well

Number of wells

The cell suspension volume needed is larger than your final well volume — dilute your stock first or use a smaller well volume.

Suspension per well

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Media per well

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Total suspension needed

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Total media needed

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Media supplements (% v/v)

Complete media from a base volume and up to two supplements added by percent — FBS, Pen/Strep, growth factors, whatever you're adding.

Final media volume (mL)

Supplement 1 name

Supplement 1 (% v/v)

Supplement 2 name (optional)

Supplement 2 (% v/v)

Your supplement percentages add up to 100% or more — there's no room left for base media.

Supplement 1

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Supplement 2

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Base media

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